Pherastar fsx microplate reader for hts bmg labtech. The pherastar fsx is the new reference multimode reader for highthroughput screening, combining the highest sensitivity with the fastest read times. Results we performed a dual shot, dual concentration primary screen on a 10,000 compound, kinase. Thanks to the unique optic module system, simultaneous dual emission, uvvis spectrometer, and dedicated alphascreen and trf lasers, whatever your application, the pherastar fsx will perform it. As 1536well microplates were introduced to high throughput screening hts, adoption was not immediate.
Design and validation of a homogeneous timeresolved. The pherastar fsx microplate reader combines unmatched speed, performance. Pherastar fs offers wide range of read outs like fluorescence, fret, luminescence etc. See if your microplate reader has the most recent software. Bmg labtech pherastar fs operating manual 20090922 0471b0001a 726 2 overview 2. Bmg labtech pherastar fs operating manual 20090922 0471b0001a 326 the pherastar fs is a multifunctional microplate reader that can perform a wide variety of applications for fluorescence intensity, fluorescence polarization, timeresolved fluorescence, htrf, luminescence, alphascreen and full spectrum absorbance. In this video you will learn how to install the software needed to control your microplate reader. Pherastar modules archives page 2 of 8 bmg labtech. This article clarifies how you can use htrf plate reader from bmg. Pherastar fsx control and mars data analysis software the pherastar fsx software package provides an extensive range of possibilities for both test protocol definitions and data analysis and is. High quality bmg labtech microplate readers range from singledetection mode plate readers up to multimode plate readers.
The newest bmg labtech control software and mars data analysis package for pherastar, pherastar plus, pherastar fs, and pherastar fsx class readers. The pherastar, pherastar plus and omega microplate readers have received the. The calculated ic50 24 nm value complies with the literature. Amongst the things highlighted at slas2012 were the pherastar fs, the new goldstandard for hts instruments.
Measurement conditions should be configured in the instrument control software according to the following parameters. Microplate stacker the stacker is an ideal solution for midthroughput labs. The reader control software is an intuitive and easytounderstand interface for. All software is 21 cfr compliant, designed to run on windows operating systems windows 7 and higher and will be delivered via cd, download link, or usb. Using clariostar and pherastar fs to monitor ligand binding. Moreover, the pherastar fsx comes with three integrated microplate barcode readers capable of reading the east, west and south side of a microplate. Automation of in vitro htrf assays on the labcyte access. The fluorescence of serial dilutions of lumi4 tblabeled antibodies in krebs buffer was simultaneously measured in the same microtiter plate, and the 665 nm emission was. The pherastar fs is driven by bmg labtechs wellknown reader control software and data evaluation is performed by bmgs exclusive microplate analysis and reduction software, mars. Pherastar fsx multimode microplate reader from bmg. The htrf optic module used 33080 nm excitation and 62010 and 66580 nm emission filters, along with a 650 nm cutoff dichroic mirror with matched dual photomultiplier.
Apr 01, 2011 the fluorescence of lumi4 tb and d2 were measured respectively at 620 and 665 nm 60. The shareware available here for downloading is fully functional. This further reduces perwell costs and enables larger screens to be performed in a cost. Fs is optimized for the most demanding hts assays including htrf. Timeresolved fluorescence resonance energy transfer tr. The user can always find the best combi nation of sensitivity and speed by.
Htrf terbium cryptate donor red acceptor readout pherastar. Bmg labtechs unique and intuitive mars data analysis software is a. Now instruments with 1536well microplate capability are a mandatory consideration for. The htrf how they run fast pace handicapper is a pacebased handicapping program.
Adpglo, lanthascreen, htrf camp and ipone, lance, alphascreen. Htrf assay setup for bmg labtech pherastar fs multimode. Cellular and biochemical htrf assays measured in 1536well. It is fully compliant with fda regulation 21 cfr part 11. Government prime contract or subcontract, software is delivered and licensed as commercial computer software as defined in dfar 252. Toward the identification of viral capmethyltransferase. The timeresolved fluorescence mode was explored using the htrf reader control. Results we performed a dual shot, dual concentration primary screen on a 10,000 compound, kinase focused library against two kinase targets of interest at 12m and 48m. The pherastar fs combines fast read times necessary for highthroughput screening and the sensitivity to read small volumes.
Additional system requirements pherastar, pherastar plus, optima series, nephelostar, novostar. All optic modules are easily exchangeable, barcoded, and are automatically selected by the. Oct 10, 2016 this video will give you the basics on setting up your pherastar fs multimode plate reader to run cisbio htrf assays. Used microplate readers biodirect used lab equipment. Pherastar fsx hts microplate reader from bmg labtech. The pherastar fs is the ultimate multimode microplate reader for hts labs, core. Please use one of the following formats to cite this article in your essay, paper or report. Detection and quantification of tau aggregates in brain tissue. Thanks to the unique optic module system, simultaneous dual emission, uvvis. Plates were read on a bmg labtech pherastar fs microplate reader. Bmg labtech pherastar fs operating manual 20090922 0471b0001a 326 the pherastar fs is a multifunctional microplate reader that can perform a wide variety of applications for fluorescence. Easytohandle, assayoptimized optic modules contain applicationspecific filters, mirrors, dichroics, andor.
Pherastar fsfsx readers equipped with trf detection. The timeresolved fluorescence mode was explored using the htrf reader. Mars evaluation software, including a 4parameterfit function, was used for. Reply to antipsychotics with similar association kinetics. Control software bmg labtech 28 0470f0026a 20100315 this manual was designed to guide pherastar users through the software features. The microplate reader pherastar fsx from bmg labtech can. Pherastar fs and the htrf ratio was plotted versus log sah concentration. The pherastar fsx is our latest, most sensitive multimode reader, combining this sensitivity with fast read times. Htrf readout can be achieved by pherastar fs after the installation of the htrf dedicated optical block which includes the optimized excitation and emission filters, the dichroic mirror and the beam splitter. Bmg labtech pherastar modules can be used in any pherastar microplate reader. This video will give you the basics on setting up your pherastar fs multimode plate reader to run cisbio htrf assays. Although these instructions were carefully written and checked, we cannot accept responsibility for problems encountered when using this manual. Better sensitivity fi and fp plate format pherastar fs pherastar fsx conditions 384 0.
The mars wizard creates an easy stepbystep calculation of a standard curve and the mars template manager is an excellent tool for building complex data processing. Thanks to the unique optic module system, simultaneous dual emission, uvvis spectrometer, and dedicated alphascreen and trf lasers, the pherastar fsx will perform your assay with ease and speed, even in 3456well microplates. The pherastar fsx is our most sensitive multimode reader for highthroughput screening, combining this sensitivity with fast read times. Easy selection of one of the four preset configurations for a specific application segment provides a fast solution to solve your. Additional system requirements pherastar fsx, pherastar fs, clariostar, omega series. Easytohandle, assayoptimized optic modules contain applicationspecific filters, mirrors, dichroics, andor polarizers, and are automatically recognized by the reader. Right side of the pherastar fs, showing the reagent injection system press the button on the grip and the optic module door can be slid aside. The gold standard for highthroughput screening pherastar fs assay speci. Discontinued microplate reader pherastar fs from the front view. Thanks to the unique optic module system, simultaneous dual emission, uvvis spectrometer, and dedicated alphascreen and trf lasers, whatever your application, the pherastar fsx will perform it with ease and speed, even in 3456well microplates. Ic 50 htrf for 1159, 597, 194 were not determined due to.
The fluorescence of lumi4 tb and d2 were measured respectively at 620 and 665 nm 60. This video demonstrates how to change and add excitation and emission filters within the software for a bmg labtech microplate reader. Htrf output provides an organized display of the matchups in todays race. In single flash mode, the pherastar fs can read a 1536well plate in 27 seconds, making the pherastar fs. Oct 27, 2015 please use one of the following formats to cite this article in your essay, paper or report. The performance and features of the pherastar fs are presented in this. Mars evaluation software, including a 4parameterfit function, was used for curve fit and ic50 determination. Plates were read on a pherastar fs plate reader bmg labtech, cary, nc equipped with alphalisa and htrf optic modules, as well as alphascreen and htrf lasers for excitation. Signal detection was performed on a pherastar fs bmg labtech, offenburg, germany using standard htrf settings and experiments were analyzed by. Development of homogeneous htrf insulin assay using pherastar fs microplate reader. The gold standard for hts microplate readers, the pherastar.
The pherastar fs can accommodate up to six optic modules. Additional system requirements pherastar fsx, pherastar fs, clariostar, omega series, spectrostar nano and nephelostar plus. The results presented in table 1 indicate that the ic 50 htrf vary from 0. Htrf assay setup for bmg labtech pherastar fs multimode plate. Timeresolved fluorescence resonance energy transfer trfret. Reliable measurement of htrf assays in 1536well format. The pherastar fsx, clariostar, polarstar omega and fluostar omega are multidetection microplate readers that are all htrf certified by cisbio. The first inaugural slas took place on february 68th in san diego, california with bmg labtech being a proud diamond sponsor of the event. Sparks dedicated, patentpending high speed monochromator is designed to set new standards for absorbance measurement speed and accuracy.
Amongst the things highlighted at slas2012 were the. The control software allows users to define instrument parameters and test protocols. The low volume increment of the echo liquid handler enabled an investigation of further miniaturization of htrf assays to as low as 2. Fluostar pherastar fs novostar nephelostar stacker find our microplate readers on. Schematic layout of the simultaneous dual emission sde optical pathway incorporated in the pherastar fs. The modules listed here have been extensively tested by our scientists for the applications shown and are optimized those assays. Using the mars data analysis software, the ic50 value calculated from. The pherastar fs has highend performance in all plate. Singledetection mode plate readers multimode plate readers. The measurement conditions should then be set up in the instrument software according to the following indications. M, among which 4 have an ic 50 htrf higher than 75. The 4parameter fit was performed with the mars data analysis software and was.
Analysis of highthroughput screening data has shown two trends, classic hts platforms have not delivered the number of expected drug leads as once thought and the more. Pherastar fsx no longer worry about installing filters and dichroics. Measurement was done on the pherastar fsx using the laser in. Data collection with the bmg pherastar fs reader demonstrates consistent assay performance at the lower volumes. Htrf ipone assay performed on the pherastar fs microplate. Drug hits in the ipone htrf say ell clariostars lvf monochromator. The pherastar, pherastar plus and omega microplate readers have received the lanthascreen certified plus designation from invitrogen which ensures that these readers are validated to strict standards in instrument setup and assay perform. Htrf experiments were performed on a pherastar fs reader bmg labtech, offenburg, germany using dedicated htrf settings excitation wavelength 337 nm. Data collection with the bmg pherastar fs reader demonstrates. Software updates for your microplate reader bmg labtech.
Pherastar fs is the next generation highthroughput. Pherastar fs control and mars data analysis software the pherastar fs software package provides an extensive range of possibilities for both test protocol definitions and data analysis. Delta f% calculation for htrf z calculation userdefined formula generator fda 21 cfr part 11 compliant multiuser software license included overlay plot of esterase catalysed pnpa reactions at different concentrations. Plate format pherastar fs pherastar fsx conditions 384 0.
Welcome to htrf software thoroughbred handicapping software. Seven certifications of bmg labtech microplate reader. All optic modules are easily exchangeable, barcoded, and are automatically selected by the pherastar fs for the appropriate assay. Pherastar fs is the next generation highthroughput screening. It offers high sensitivity and using this reader, it provides higher signal window as compared to others. The certification is given to microplate readers that meet cisbios expectations in terms of sn, df%, cv%, sensitivity and dynamic range. Pherastar fsx multimode microplate reader from bmg labtech. Mar 08, 2017 the 4parameter fit was performed with the mars data analysis software and was.
Twelve to 14point, halflog dilution enzyme titration curves for g9a and set79 were prepared in echo. Optic module, htrf, fi 405 530 460, lanthascreen, fp rhodamine, fi 405 530 460. These experimental evaluations show that the pherastar fs can produce high quality data for the alphascreen assay technology. While an integrated fluorophore library in the control software simplifies assay. Pherastar plus hts microplate reader from bmg labtech. Evaluate assay performance using z, delta f% for htrf, signalto blank. The user can always find the best combi nation of sensitivity and speed by choosing the number of flashes. The rubystar was the first and only laserbased htrfdedicated reader on the market. Cell lines see table 1 expressing the gpcr target of interest were used for measuring ip1 production by stimulation of the ligand. The future of highthroughput screening with the pherastar fs. Thanks to the unique optic module system, simultaneous dual. Additional system requirements pherastar fsx, pherastar fs, clariostar. Unparalleled sensitivity in the widest range of applications.
This article looks at using two htrf assays from cisbio bioassays. Bmg labtech also featured the pherastar fs in a second tutorial at the slas2012, new hts tools for discovering low affinity inhibitors in primary and secondary screens. Development of a rapid insulin assay by homogenous time. Htrf ratios obtained for the ipone assay with the pherastar fs microplate reader.